RNAseq of Melanoplus sanguinipes: first instar whole insect - SRA

SRX24725908: RNAseq of Melanoplus sanguinipes: first instar whole insect
1 ILLUMINA (Illumina HiSeq 2500) run: 31.3M spots, 7.9G bases, 2.8Gb downloads

Design: Total RNA was extracted by homogenizing in TRIzol reagent according to the manufacturer's protocol (Invitrogen). Total RNA was resuspended in sterile DEPC-treated H2O and quantified by measuring absorbance at 260 and 280 nm using a Nanodrop spectrophotometer (Fisher-Thermo). RNA was run through the Micro poly-A purist column (Fisher-Thermo) to select for polyadenylated transcripts. The quality of the RNA was assessed using an RNA 6000 Nano Kit and a Bioanalyzer 2100 instrument (Agilent). Illumina sequencing libraries were synthesized and sequencing performed at the National Research Council, Saskatoon, Canada

Submitted by: Agriculture Canada

Study: RNAseq of the AAFC Migratory Grasshopper colony

PRJNA1117477 • SRP510303 • All experiments • All runs

show Abstracthide Abstract

To generate RNAseq data for life stages of the Migratory Grasshopper (Melanoplus sanguinipes)

Sample:

SAMN41571455 • SRS21448656 • All experiments • All runs

Organism: Melanoplus sanguinipes

Library:

Name: Ms-1st

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq